- Visibility 59 Views
- Downloads 14 Downloads
Comparison of conjunctival microflora in patients undergoing cataract surgery
- Author Details:
-
Gautam Paul
-
Debadatta Dhar
-
Ankita Narula
-
Abhisek Mandal
-
Tshering Wangchuk Bhutia
Purpose: To isolate the bacterial flora from the conjunctival sac in patients undergoing cataract surgery and to determine the antibiotic sensitivity pattern of these organisms.
Methods: A total of 500 cases both men and women were included in this study. Specimen were taken 1 day prior to the surgery from the conjunctival sac of the patients and then cultured for isolation of the bacteria along-with antibiotic sensitivity testing if any culture came positive. The percentage of bacterial isolates and the drug resistant isolates were compared. The study was conducted for a period of 18 months after obtaining a written and informed consent from all the patients. All forms of cataract in all age groups with the absence of any associated ocular infection were included in this study. Patients with prior history of any ocular infectious diseases and traumatic cataracts were excluded from this study.
Results: Out of the 500 cases, 61 (12.2%) had positive cultures. The most frequently isolated bacterium was the Staphylococcus aureus found in 44(72.13%) cases, followed by coagulase negative Staphylococcus (CoNS) from 11(18.03%) cases, both showing high sensitivity to cefpodoxime (65%), cefuroxime (67%), gentamicin (74%), ciprofloxacin (72%) and vancomycin (83%), amoxycillin and clavulanic acid (53.5%) and methicillin (59%). Methicillin resistant strains were noted in 19.36% eyes.
Conclusion: The most frequent bacterium in the conjunctival flora is the Staphylococcus aureus with high susceptibility rates to cefpodoxime, cefuroxime, gentamicin, vancomycin, ciprofloxacin and augmentin.
Keywords: Antibiotics, Cataract surgery, Conjunctival flora, Sensitivity.
References
- Javitt JC, Kendix M, Tielsch JM, Steinwachs DM, Schein OD, Kolb MM, et al. Geographic variation in utilisation of cataract surgery. Med Care. 1995;33(1):90-105.
- Ciulla TA, Starr MB, Masket S. Bacterial endophthalmitis prophylaxis for cataract surgery: an evidence-based update. Ophthalmology. 2002;109(1):13-24.
- Kanellopoulos AJ, Dreyer EB. Postoperative infection following current cataract extraction surgery. Int Ophthalmol Clin. 1996;36(3):97-107.
- Speaker MG, Milch FA, Shah MK. The role of external bacterial flora in the pathogenesis of acute endophthalmitis. Ophthalmology. 1991;98:639–49.
- Reza M, Madani H, Ghaderi E. Conjunctival bacterial flora and antibiotic resistance pattern in patients undergoing cataract surgery. Pak J Med Sci. 2008;24:581–5.
- Cham TL, Valenton MJ, Lim R. Ocular bacterial flora and antibiotic sensitivity among Filipino patients undergoing routine cataract surgery. Philipp J Ophthalmol. 2010;34:19–22.
- Dickey JB, Thompson KD, Jay WM. Anterior chamber aspirate cultures after uncomplicated cataract surgery. Am J Ophthalmol. 1991;112(3):278-82.
- Samad A, Solomon LD, Miller MA, Mendelson J. Anterior chamber contamination after uncomplicated phacoemulsification and intraocular lens implantation. Am J Ophthalmol. 1995;120(2):143-50.
- Snyder-Perlmutter LS, Katz HR, Melia M. Effect of topical ciprofloxacin 0.3% and ofloxacin 0.3% on the reduction of bacterial flora on the human conjunctiva. J Cataract Refract Surg. 2000;26(11):1620-5.
- Graves A, Henry M, O'Brien TP, Hwang DG, Van Burskirk A, Trousdale MD. In vitro susceptibilities of bacterial ocular isolates to fluoroquinolones. Cornea. 2001;20(3):301-5.
- Garcia-Sáenz MC, Peral Ortiz de la Torre MJ, De Castro Liébana M, Jiménez Martínez E, García Sánchez JE, Fresnadillo Sánchez MJ. Flora conjuntival según edades. Arch Soc Esp Oftalmol. 1999;74(7):379-84.
- Locatelli CI, Kwitko S, Simonetti AB. Conjunctival endogenous microbiota in patients submitted to cataract surgery. Braz J Microbiol. 2003;34(3):203-8.
- Han DP, Wisniewski SR, Wilson LA, Barza M, Vine AK, Doft BH, et al. Spectrum and susceptibilities of microbiologic isolates in the Endophthalmitis Vitrectomy Study. Am J Ophthalmol. 1996;122(1):1-17.
- Pinna A, Zanetti S, Sotgiu M, Sechi LA, Fadda G, Carta F. Identification and antibiotic susceptibility of coagulase negative staphylococci isolated in corneal/external infections. Br J.
- Miño de Kaspar H, Koss MJ, He L, Blumenkranz MS, Ta CN. Antibiotic susceptibility of normal conjunctival bacteria. Am J Ophthalmol. 2005;139:730–3. Gautam Paul et al. Comparison of conjunctival microflora in patients undergoing cataract surgery Indian Journal of Clinical and Experimental Ophthalmology, October-December, 2017; 3(4): 450-453 453
- Arantes TE, Cavalcanti RF, Diniz Mde F, Severo MS, Lins Neto J, Castro CM. Conjunctival bacterial flora and antibiotic resistance pattern in patients undergoing cataract surgery. Arq Bras Ophthalmol. 2006;69:33–6.
How to Cite This Article
Vancouver
Paul G, Dhar D, Narula A, Mandal A, Bhutia TW. Comparison of conjunctival microflora in patients undergoing cataract surgery [Internet]. Indian J Clin Exp Ophthalmol. 2017 [cited 2025 Oct 26];3(4):450-453. Available from: https://doi.org/
APA
Paul, G., Dhar, D., Narula, A., Mandal, A., Bhutia, T. W. (2017). Comparison of conjunctival microflora in patients undergoing cataract surgery. Indian J Clin Exp Ophthalmol, 3(4), 450-453. https://doi.org/
MLA
Paul, Gautam, Dhar, Debadatta, Narula, Ankita, Mandal, Abhisek, Bhutia, Tshering Wangchuk. "Comparison of conjunctival microflora in patients undergoing cataract surgery." Indian J Clin Exp Ophthalmol, vol. 3, no. 4, 2017, pp. 450-453. https://doi.org/
Chicago
Paul, G., Dhar, D., Narula, A., Mandal, A., Bhutia, T. W.. "Comparison of conjunctival microflora in patients undergoing cataract surgery." Indian J Clin Exp Ophthalmol 3, no. 4 (2017): 450-453. https://doi.org/