Indian Journal of Clinical and Experimental Ophthalmology

Print ISSN: 2395-1443

Online ISSN: 2395-1451

CODEN : IJCEKF

Indian Journal of Clinical and Experimental Ophthalmology (IJCEO) is open access, a peer-reviewed medical journal, published quarterly, online, and in print, by the Innovative Education and Scientific Research Foundation (IESRF) since 2015. To fulfil our aim of rapid dissemination of knowledge, we publish articles ‘Ahead of Print’ on acceptance. In addition, the journal allows free access (Open Access) to its content, which is likely to attract more readers and citations of articles published in IJCEO. Manuscripts must be prepared in more...

Article type

Case Series


Article page

117-120


Authors Details

Parthasarathi Roy*, Pratip Kundu, Ardhendu Kumar Maji


Article Metrics


View Article As

 


Downlaod Files

   






Article statistics

Viewed: 625

PDF Downloaded: 261


A clinical case series of Acanthamoeba keratitis diagnosed by a simple technique of detection of motile trophozoites in wet mount preparation


Case Series

Author Details : Parthasarathi Roy*, Pratip Kundu, Ardhendu Kumar Maji

Volume : 9, Issue : 1, Year : 2023

Article Page : 117-120

https://doi.org/10.18231/j.ijceo.2023.022



Suggest article by email

Get Permission

Abstract

Acanthamoeba keratitis is diagnosed fairly late because of lack of early clinical suspicion and unavailability of simple and effective technique for detection of the protozoa in ordinary laboratory settings. The detection of motile trophozoites in wet mount preparation of corneal scraping is probably the best confirmatory test of Acanthamoeba keratitis in ordinary laboratory settings. However, it is not easy to demonstrate motile trophozoites given the fact that the trophozoites rapidly encyst outside living tissue. We employed a protocol of preparation and transport of the wet mount to the nearby laboratory within fifteen minutes whereby optimum chance of demonstration of motile trophozoites was possible. We had been successful to detect the typical amoeboid movement of acanthamoeba in three cases. Healing of ulcers were achieved by an appropriate therapy in all three cases. Visual recovery was good in two out of three cases. To the best of our knowledge, the present case series is the first one in English literature where diagnosis of acanthamoeba keratitis is based solely on detection of motile trophozoites in direct wet mount examination.
Key Messages: A careful history and an incisive look into the corneal lesion revealed some clue to suspect the acanthamoeba etiology. Simple laboratory technic employing wet mount microscopy rapidly confirmed the diagnoses. Healing of ulcers were achieved in all three cases by instillation of 0.02% chlorhexidine eye drop. Visual recovery was good in two out of three cases.
 

Keywords: Acanthamoeba keratitis, Wet mount microscopy, Motile trophozoites.


How to cite : Roy P, Kundu P, Maji A K, A clinical case series of Acanthamoeba keratitis diagnosed by a simple technique of detection of motile trophozoites in wet mount preparation. Indian J Clin Exp Ophthalmol 2023;9(1):117-120

This is an Open Access (OA) journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.